Tagged Expression Vectors
OriGene offers genome-wide TrueORF clones for human, mouse, rat, and viral genes. Each TrueORF clone contains a sequence verified ORF in a pCMV6-Entry or pCMV6-AC-GFP vector, and can be easily shuttled via PrecisonShuttle system. The PrecisionShuttle system is a simple 'cut-and-paste' procedure, that enables you to shuttle your clone of interest into any of the destination vectors. With the wide selection of destination vectors, TrueORF clones are ready-to-use in many downstream studies.
The destination vectors offer choices of:
- Epitope tags for protein purification and detection
- Fluorescent protein tags for visualization
- Various drug selection markers for stable cell line establishment
- Lentiviral, and AAV based expression vectors for virus production
These vectors could be ordered as stand-alone products, or you could take advantage of OriGene's custom shuttling service where OriGene will subclone the ORF insert of your interest from our TrueORF clone into any of the destination vectors.
Lentiviral Vectors
Epitope tagging
FP tagging
Selection markers
Lenti
AAV
Bacterial expression
The new E.coli destination vectors expand the utility of 37,000 TrueORF clones for protein production in bacteria. | |
Expression Validation | Product Manual |
Peptide sequence of the DDK-tag (Flag®): N-DYKDDDDK-C
Flag® is a registered trademark of Sigma-Aldrich